- as Peter Parker / Spider-Man (Voice)
- as Eddie Brock / Venom (Voice)
- as Cletus Kasady / Carnage (Voice)
- as Doctor Octopus (Voice)
- as Felicia Hardy / Black Cat (Voice)
- as Stan Lee (Voice)
- as Uatu the Watcher (Voice)
- as Quentin Beck / Mysterio (Voice)
- as Mac Gargan / Scorpion (Voice)
- as Hostage (Voice)
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