- as Dr. Leonard Leakey Hofstadter
- as Amy Farrah Fowler
- as Dr. Dr. Sheldon Lee Cooper
- as Bernadette Rostenkowski
- as Penny
- as Wil Wheaton
- as Howard Joel Wolowitz, M.Eng.
- as Dave Gibbs
- as Dr. Rajesh „Raj“ Ramayan Koothrappali
- as Mike Rostenkowski
- as Adam Nimoy
- as Mr. Spock (Archive footage)
- as Captain James T. Kirk (Archive footage)
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